sugar cane wax Extract Octacosanol Policosanol

 

Brief

Policosanol, a natural compound derived from sugar cane wax,has shown promising results in clinical studies (primarily performed in cuba) and is well tolerated.Since it is derived from a food a source,policosanol is classified as a nutraceutical ,or natural product.Commercial products are derived from a variety of different source (sugar cane wax, rice bran wax, beeswax,)but it is important to note that the sugar cane wax derivative was used in the Cuban studies.

 

Though octacosanol has been shown to individually reduce cholesterol and triglyceride,there is no doubt that the blend of the alphatic spectrum,as found in sugar,rice and beeswax,is superior to an isolated aliphatic alcohol.and ratio are different.Therefore,it is sure that the treatment effects of policosanol from different sources are not the same .For example,some published studies from Cuba sugar that beeswax products may be useful for the ulcers treatment,but not for reducing cholesterol or treating intermittent claudication.

Studies now show that at least contents of octacosanol, docosanol and triacontanol are different in policosanol from sugar,rice and beeswax.   

 

Benefits:

Effects of Policosanol supplementation have been proven by studies of humans and animals.

·Effective LDL reduction;

·Increase HDL levels;

·Amelioration of coronary heart disease;

·Increased utilization (hence elimination)of visceral fat;

·Improved muscle endurance;

·Helps intermittent claudication.

 

Claims as following that have been made for policosanol are debatable.

·Improved sexual activity and libido;

·Increased production of sex hormones;

·Improved physical strength and muscle reflexes;

·Prevention of muscle spasms;

·Reduced lipid peroxidation(rancidity);

·Improved resistance to stress;

·Raising of basal metabolism;

·Increased hypotensive(blood-pressure-lowering)effect of beta-blockers;

·Protection of endothelial(blood vessel)cells.

General

Policosanol is a mixture of primary long-chain aliphatic(non-ring)alcohols.The main ingredient in policosanol is octacosanol, but as the name implies,policosanol is actually a mixture of many (hence the prefix”poll”) alcohols,including hexacosnol,triacontanol and others.Policosanol is refined from the form of waxes that are prevalent throughout nature.Sources used in dietary supplement industry include sugar cane, rice brain, beeswax, broccoli, spinach,alfalfa and oats.sometimes, a little amount of individual alcohol like Heptacosanol and Nonocosanol exists in waxes.generall speaking,effective components can be analyzed including Tetracosanol, Hexacosanol, Octacosanol, Triacontanol and Dotriacontanol.

Policosanol is a group of active compounds composed of predominantly five higher primary aliphatic alcohols-Tetracosanol,Hexacosanol, Octacosanol,Triacontanol and Dotriacontanol.

Source:Sugar cane wax.

Constituents

 

 

Pharmacology:

Different with other lipid reducing drugs such as Lovastatin,policosanol inhibits the cholesterol biosynthesis during the consumption of acetic acid and occurrence of mevalonic acid,but not to carry out the direct inhibition to HMG-CoA, the key enzyme of cholesterol biosynthesis.

 

Policosanol has demonstrated potent effect on reducing the liquid peroxidation in the serum,specially on inhibiting lipid peroxidation induced by CCL₄ in rat’s liver microsome,as well as the oxidation of very low-density lipprotein (VLDL) and lipid peroxidation of rat lipprotein caused by Cu²⁴ thus, it can bring potential accessorial therapeutic benefit to atherosclerotic damages.

 

Blood vessel endo thelial tonic function also goes to polico sanol.It lowers endothelial counts in the serum of spontaneous hypertensive rats with endothelial damages induced by citrate,and rabbit with such damages caused by operation instruments.,

 

Safety

Policosanol appears to be safe.LD₃₀>5000mg/kg (oral administration in rats). No obvious side effects were observed in most studies,which are theoretically possible to occur in the case of being allergic with sugarcane.

 

In addition,because policosanol can thin the blood at least as much as aspirin,those taking blood thinners (Coumadin ,Warfarin) or anti-platelet medications (Heparin) should consult with their healthcare provider prior to taking policosanol.

 

Our manufacture

In practice,we adopt the supercritical co₂ extraction for the production of policosanol from sugarcane.Little solvent and the advantages of the technology itself make it possible to protect the effective constituents.So, the ultimate protection of various effective constituents and the minimum solvent residue make the greatest particularities of our company.

Specification

OCTA-90( C28alcohol ≥ 90%)

OCTA-60( C28alcohol ≥ 60%)

OCTA-50( C28alcohol ≥ 50%)

OCTA-25( C28alcohol ≥ 25%)

OCTA-12( C28alcohol ≥ 12%)

 

1.  Chromatoqraphy conditions

Column:2.5% OV-17 +2.5% SE-30,

Coated on Chromosorb WAWDMCS (80-100 MESH),3m×3mm.

Detector:FID                      Injection volume:2μL

Sensitivity:10’                    Injector temperature:310°C

Carrier(N2):180Kpa      Detector temperature:320°C

H₂:65kpa                Column temperature:300°C

Air:40Kpa

 

1.Solution preparation  

Preparation of standard solution

Accurately weigh about 20mg octacosanol reference into 10ml volumetric flask,and add chloroform,then sonicate to dissolve and dilute to volume.

 

 

Preparation of test solution

Accurately weigh moderate sample into 10Ml volumetric flask,add about BmL chloroform and sonicate to extract for 5min at 50°c,then cool to room temperature.Dilute to volume with chlo-roform to calibrate ,and shake fully to obtain the test solution.

 

2.  Determination of sample content

Under above chromatographic conditions ,conduct the determination at the stability of apparatus and baseline. The retention time of octacosanol is about 11min ,Calculate the content with external standard method.

 

Certificate of Analysis-Policosanol(OCTA-60

Source:Sugar cane wax

Assay	Octacosanol	≥ 60%
	Appearance	White fine powder
	Odor	Characteristic
	Loss on drying	≤0.5%
	Residue on ignition	≤0.5%
Physical characteristic	Particle size	100% thru 80 mesh
	Melting point	Report Data
	Solubility	80.2-81.3°C
		Soluble in hot Chloroform,Petroleum Ether,
		Ethanol,Acetone,Ethyl ether,Benzene,
		Toluene. Not soluble in water
Microbiology	Total plate count	<1000cfu/g
	Yeast &Mold	<100cfu/g
	E.Coli	Absent
	Salmonella	Absent
Residue	Solvent(Petroleum Ehter)	70-150ppm
	Heavy Metal	As:10-20ppm
		Pb:1-2ppm
	Pesticide	Absent